0 Comments
e) Droplet overloading boosts the percentage of droplets filled with nuclei from 16.4% (obtained for the maximum loading concentration of the standard Chromium protocol) to 95.5% (obtained for 100-fold overloading using 1.53 million nuclei per channel). d) Representative images of droplets containing between one and ten nuclei, showing the overloading of a standard microfluidic droplet generator (10x Genomics Chromium). c) Detailed method design of scifi-RNA-seq. b) scifi-RNA-seq, which uses preindexing and droplet overloading to boost the throughput of droplet-based scRNA-seq. Scifi-RNA-seq combines preindexing of whole transcriptomes with droplet-based scRNA-seqĪ) Standard droplet-based scRNA-seq, where cells are loaded at a low concentration (limiting dilution) to avoid cell doublets, and most droplets do not receive a cell. Once the transcripts have been indexed inside the microfluidic droplets, the emulsion is broken, and the remaining steps of the library preparation are performed in bulk. All primers on a given bead carry the same barcode, which uniquely identifies the coencapsulated cell. Cells are then lysed inside the droplets, RNA molecules anneal to bead-tethered primers, and reverse transcription is performed inside the droplets. When aqueous and oil phases are combined at controlled flow rates, emulsion droplets coencapsulate individual cells with individual microbeads. In a typical droplet-based scRNA-seq experiment, the single-cell suspension is processed on a microfluidic chip, together with uniquely barcoded microbeads, reverse transcription reagents, and carrier oil ( Fig. With their high throughput, straight forward handling and consistent data quality, they have contributed to the wide adoption of single-cell RNA-seq (scRNA-seq) in many areas of basic biology and biomedical research. Microfluidic droplet generators are currently the most popular technology platform for single-cell sequencing 1– 3. We benchmarked scifi-RNA-seq on various human and mouse cell lines, validated it for primary human T cells, and applied it in a highly multiplexed CRISPR screen with single-cell transcriptome readout of T cell receptor activation. Compared to cell hashing methods, which flag and discard droplets containing more than one cell, scifi-RNA-seq resolves and retains individual transcriptomes from overloaded droplets. Compared with multiround combinatorial indexing, scifi-RNA-seq provides an easy and efficient workflow. Thereby, scifi-RNA-seq massively increases the throughput of droplet-based single-cell RNA-seq, and provides a straight forward way of multiplexing thousands of samples in a single experiment. Preindexing allows us to load several cells per droplet and computationally demultiplex their individual expression profiles. The scifi-RNA-seq assay combines one-step combinatorial preindexing of entire transcriptomes inside permeabilized cells with subsequent single-cell RNA-seq using microfluidics. To enable cost-effective single-cell sequencing for millions of individual cells, we developed ‘single-cell combinatorial fluidic indexing’ (scifi). Cell atlas projects and high-throughput perturbation screens require single-cell sequencing at a scale that is challenging with current technology. The MPV is also longer than the Crysta and accommodates up to 8 adults with greater comfort. Synonymous with 7 seater cars in India, the 3rd generation of the Innova is also the closest the MPV has come to driving like a normal car, courtesy of its monocoque chassis, front-wheel drive, and a petrol-hybrid powertrain. Transmission: 6-speed manual, 6-speed automatic.Specifications & Key Features of Mahindra XUV700 And while it’s no longer the only one in its segment to offer ADAS, the XUV700 is still one of the few cars in India with a 5-star Global NCAP rating. Even more impressive is the XUV700’s driving dynamics which put it closer to a car than an SUV, thanks to the optional all-wheel drive. Offering petrol and diesel engines, Mahindra’s flagship three-row SUV boasts sub-10-second 0-100 kmph times. Jack of all trades, master of quite a few! The XUV700 is one of the best cars in India with its tech package, capable powertrains, and proven safety. Engine: 1.5-litre turbo diesel (76 PS & 210 Nm).Specifications & Key Features of Mahindra Bolero Hardly a wonder then that the Bolero is by far the most popular in rural markets. The Bolero is also remarkably easy to maintain, thanks to affordable spare parts, Mahindra’s wide service network, and the MUV’s rather simple mechanicals. The rugged SUV can march on unfazed by potholes, bad patches, broken roads, and even no roads. The Mahindra Bolero is one of the hardiest 7 seater cars in India today, thanks to its ladder-frame chassis, commercial vehicle-grade suspension, and rear-wheel drive. 8-inch infotainment with Apple CarPlay/Android Auto.Specifications & Key Features of Toyota Innova Crysta Armed with Toyota’s legendary reliability, the Crysta is also one of the few three-row cars in India that can comfortably seat 7 or 8 people with 300 litres of boot space to spare. The Crysta is also the only MPV in India with a ladder-frame chassis and rear-wheel drive, which make life easier when tackling bad road surfaces. It’s powered by a bulletproof 2.4-litre diesel engine paired with a 5-speed manual transmission, a combination known for its excellent driveability and fuel efficiency. Transmission: 5-speed manual, 6-speed automaticĪ byword for 7 seater cars in India, the Innova Crysta is on sale even after the Hycross’s launch and it’s easy to see why.Lastly, the Ertiga is also the only 7 seater car in India to offer a CNG variant Specifications & Key Features of Maruti Suzuki Ertiga It also aids acceleration, helping the Ertiga gather momentum even with seven on board. The tech automatically turns the engine off at idle, only to restart it when the clutch pedal is pressed. While it doesn’t get a diesel engine, this three row MPV is among India’s most efficient cars with its mild-hybrid petrol engine. Things working in its favour include enough space to seat seven adults, reliable underpinnings, and Maruti’s trust factor. The Maruti Suzuki Ertiga is the best 7 seater car in India in terms of sales. Says Lyndsey Murray, a certified sex therapist and the owner of Relationship Matters Therapy, a practice that helps individuals and couples improve their relationships and sex lives, “Usually I find there is a link between a certain food and particular memories or fantasies someone may have that are associated with that food. This play can be arousing in the way they heighten the senses of smell, taste, and feel. This can include drizzling melted chocolate or whipped cream over the body and licking it off or using frozen fruits like cherries or strawberries to create cooling sensations along the neck, breasts, or thighs. Fruits and vegetables shaped like penises can be incorporated into food play, with zucchinis, cucumbers, and bananas being used for vaginal or anal penetration, but the fetish can involve a more sensual, aromatic incorporation of food. Other foot fetish activities include giving pedicures and smelling the feet or shoes that said feet reside in throughout the day.įood play, also known as sitophilia, refers to a kind of fetish in which individuals are turned on by food in an erotic setting. Humiliation can also play a strong role in a foot fetish: The fetishist may want feet shoved in their face and mouth, or to have their partner walk all over them, touting how filthy their feet are and how pathetic the fetishist is to enjoy such an activity. But like all kinks, foot fetishes exist on a spectrum. So even if you don’t have a foot fetish, having a partner with one can be extremely enjoyable as it means there could be foot massages galore in store for you. They often want to engage in foot worship, in which they treat their partner’s foot like a holy object: kissing, caressing, and massaging it. Someone with a foot fetish is literally turned on by feet-thinking about them, touching them, seeing them-and could potentially get off on everything feet. This is one of the most common fetishes out there, especially for heterosexual men. This play needs to be done with extreme caution and care.” Just because you saw choking in porn or are interested in it does not mean you should run off and choke your partner. “It's important to address that choking during sex is extremely dangerous and should be done with caution,” says Engle. There is no truly safe way to perform erotic asphyxiation instead, those interested might try light choking, after carefully communicating consent and clear physical boundaries, but even that comes with risk. Erotic asphyxiation can result in an irregular heart rate, cardiac arrest, brain damage, and even death. This is something many of us long for: a release from control or the power to take it.” But the risks outweigh the pleasure of this kind of release. Giving someone permission to choke you, or visa versa, is allowing you to take on dominant and submissive roles in bed. “Choking highlights the giving and receiving of control. “The turn on has deep psychological implications that can give opportunity to explore different roles during sex,” says Engle. During the act, some people find pleasure in the exchange of power. Depending on whether it is performed on a partner or on oneself (autoerotic asphyxiation), erotic asphyxiation is a type of sadistic and masochistic fetish. You feel your partner’s touch on your skin more acutely, smells become more vibrant, and orgasms can even be more intense.” Blindfold sex can also be a popular kink among people who struggle with anxiety during sex, with the blindfold offering comfort and a sense of escapism.Įrotic asphyxiation, also known as breath play, is a fetish that involves limiting the supply of oxygen to the brain during sex in order to heighten arousal and intensify orgasms. “When you’re blindfolded, all of your other senses become enhanced. Plus the element of sensory deprivation can help a person’s senses, aside from sight, to be magnified. “At its core, you're giving up control to another person and allowing them to take control,” says Gigi Engle, ACS, a certified sex educator and resident intimacy expert at 3Fun, an app for open and polyamorous couples. This practice can be super arousing for some, as it necessitates an element of surrender-you need to have built trust with the partner who is not blindfolded. Blindfold sex can involve a silk tie or satin blindfold placed over the eyes, one which is close-fitting, but not overly tight. The kink involves a preference for being blindfolded during sex, having sex with someone who is blindfolded, or even having sex in complete darkness. Blindfold sex, also known as amaurophilia, is a gateway to more BDSM play. The standard-fit autonomous emergency braking (AEB) system performed well in tests of its functionality at the low speeds at which many whiplash injuries occur, with collisions avoided in most test scenarios. A geometric assessment of the rear seats also indicated good whiplash protection. Apart from several subtle exterior and interior updates, the car is now engineered above the B0+. Tests on the front seats and head restraints demonstrated good protection against whiplash injuries in the event of a rear-end collision. CAPTUR 2020 1.6 16V SCE FLEX INTENSE X-TRONIC CAPTUR 2018 1.6 16V SCE FLEX ZEN MANUAL CAPTUR 2020 1.6 16V SCE FLEX INTENSE X-TRONIC CAPTUR 2021 1.6. In May 2020, Renault Russia launched the facelifted Kaptur. Even in the more severe side pole test, protection of the chest was adequate and that of other body regions was good. In the side barrier impact, protection of all critical body areas was good and the car scored full points in this test. In the full-width rigid barrier test, protection was good or adequate for all critical body areas, both for the driver and the rear passenger. Protection was rated as good for all critical body areas for the passenger. Tive a oportunidade de passar alguns dias com o Renault Captur Intense 2020 e evidentemente gravei minhas impresses e minha opinio sincera para vocs.E a. Renault showed that a similar level of protection would be provided to occupants of different sizes and to those sitting in different positions. 1 3 The production version of the first one, based on the B platform, made its debut at the 2013 Geneva Motor Show and started to be marketed in France during April 2013. Dummy readings indicated good protection of the knees and femurs of the driver and passenger. The Renault Captur (pronounced 'Cap-turr') is a series of subcompact crossover SUVs manufactured by the French automaker Renault. The passenger compartment of the Captur remained stable in the frontal offset test. TikToker coined the term in July, explaining in a video - which has 2.3 million views and 301,800 likes - that if you want to know what boy dinner is, “go to your local supermarket at 6:30 and stand behind a single man and see what’s inside of his basket. Ve contenido popular de los siguientes autores: Overtime(overtime), ABC News(abcnews), SableServingLewks(kittykatsable), gmoney(gl00mie), Mariangela Filippone(marefils). With girl dinner going so viral, it was only a matter of time before boy dinner made its way onto the platform.īoy dinner is meant to show a stereotypical dinner that a man would eat which is satirically masculine - meaning it’s simple and barbaric. Descubre en TikTok los videos cortos relacionados con nyc subway flood. It takes just a few minutes, and you use practically no energy: That is a girl dinner. Maybe you put a plate together of some leftover slices of deli meat, fruits and cheese and crackers rather than making a full-out meal. The hashtag #girldinner has accumulated a whopping 1.7 billion views on TikTok. If you’re active on social media at all, you’ve definitely heard of “ girl dinner” - but there’s a “boy dinner,” too. I went to a birthday dinner - and fought over splitting the $4.6K bill TikToknyc flooding recovery Nicholas(rmblsti25), Cookielicious720(720lifestyle), radical leftist cousin(izabreal), NYCWired(nycwired), Assistmaintenance(assistmaintenance), House of Highlights(houseofhighlights), Ranz Sayaman(rastamanranz), Jacklyn Marval. Is ‘girl dinner’ healthy - or an eating disorder? Experts weigh inĭon’t fall prey to fake ‘girl dinner’ trend outrage, food experts say There’s a laxative shortage in the US - partly thanks to ‘budget Ozempic’ TikTok trend So it should have ended a long time ago, but when? What was the point where things started going down hill, where you sacrifice a few gems for the sake of losing a lot of crud? And what episode would serve best as a climax, a cap-stone that celebrates everything good about the show? Here are ten Simpsons episodes that would have been perfect finales. The Simpsons will keep running and running until Jean enacts his weird time is a flat circle ending which ensures that, technically, the show will continue into infinity. And yet, Fox continue to keep Barney Gumble from smothering the show with a pillow, let alone to smash a window with a water fountain and escape. Almost all of its best writers have left to do better things, Matt Groening has been gone since relatively early on, and now Harry Shearer voice of key characters such as Mr Burns, Smithers, Principle Skinner, Ned Flanders has quit, which seemed like a good excuse to end it. There are few who will argue against The Simpsons long being past its best. The Simpsons animated sitcom television show has been on-going since 1989. Whole series a continuous loop. Yes, even long-time Simpsons producer Al Jean's ideal finale for the long-running, long-suffering show basically implies that the unageing, unchanging, unhappy residents of Springfield are living in purgatory. The Yoko Ono-inspired character also collaborates on a track with Barney in which she records herself saying the phrase number 8 over and over again to a back track of burps, a parody of Ono. My idea for last episode ends with the arrival at the Christmas pageant from the beginning of first episode. Naudodami Tenor, GIF klaviatros krimo priemon, pridkite populiari Barney Simpsons animuotj GIF element prie savo pokalbi.
When times are bad or sad, think about all the things that make you happy. The bright blessed day, the dark sacred nightĪnd I think to myself what a wonderful world. Listen to Doris Day’s original recording:Īnd I think to myself what a wonderful world. Feel free to swap out the gender if you’re singing to your little boy. And it provides a great lesson about life, i.e., “whatever will be, will be.” The catchy melody makes it easy to sing. It couldn’t be a better lullaby for a child who’s filled with wonder. Each verse of the song progresses through the life of an inquisitive child (in this case, the singer) who asks their mother questions about life and love. The song went on to win an Academy Award, became Day’s signature song, and was recorded by countless artists thereafter. “Que Sera, Sera (Whatever Will Be, Will Be)”įirst introduced in 1955, “Que Sera, Sera” was made famous a year later by Doris Day in Alfred Hitchcock’s film The Man Who Knew Too Much. Till by turning, turning we come ’round right. 'Twill be in the valley of love and delight. 'Tis the gift to come down where we ought to be,Īnd when we find ourselves in the place just right, 'Tis the gift to be simple, ’tis the gift to be free Thanks to Copland, we have this beautiful tune with touching lyrics that make the perfect lullaby. He later released the song in 1950 as part of a set in Old American Songs. “Simple Gifts”īased on a Shaker tune from 1848, “Simple Gifts” wasn’t very well known outside of the Shaker community until composer Aaron Copland used the melody in his Appalachian Spring, which premiered in 1944. The winds of night so softly are sighing, It fills the sails of boats that are waiting, To learn more about how and for what purposes Amazon uses personal information (such as Amazon Store order history), please visit our Privacy Notice. You can change your choices at any time by visiting Cookie Preferences, as described in the Cookie Notice. Click ‘Customise Cookies’ to decline these cookies, make more detailed choices, or learn more. Third parties use cookies for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products. Arnold Schwarzenegger Conquer Poster for Sale by gimmethatplease. 1500x1028 Arnold Schwarzenegger Bodybuilding Wallpaper Posters And Picture'> Get Wallpaper. 1600x1000 Pretty nice Motivation Wallpaper with Quotations for You. 25.13 (20 off) motivation Arnold Schwarzenegger conquer Poster. 27.22 (20 off) Arnold Schwarzenegger Arnie Conquer Quote - Poster. This includes using first- and third-party cookies, which store or access standard device information such as a unique identifier. Schwarzenegger Conquer Poster by PixelBase Download Share on Facebook Share on Twitter. 1920x1200 Arnold Schwarzenegger Conquer Wallpaper'> Get Wallpaper. Arnold Schwarzenegger 'Conquer' Motivational Fitness Poster. If you agree, we’ll also use cookies to complement your shopping experience across the Amazon stores as described in our Cookie Notice. Add to Favourites Arnold Schwarzenegger Poster,Body Building Canvas,Arnold Schwarzenegger Conquer Motivational Inspirational Office Decor,Man Cave Wall Decor (11. We also use these cookies to understand how customers use our services (for example, by measuring site visits) so we can make improvements. Arnold Schwarzenegger Conquer - Gym Poster Quote Motivational Fitness Inspiration Mental Health - High Quality Vector Image SGD 18.93.
|
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |